Bulked-Segregant Analysis Coupled to Whole Genome Sequencing (BSA-Seq) for Rapid Gene Cloning in Maize

Gene Mapping via Bulked Segregant RNA-Seq (BSR-Seq)

Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. BSA requires access to quantitative genetic markers that are polymorphic in the mapping population. We have developed a modification of BSA (BSR-Seq) that makes use of RNA-Seq reads to efficiently map genes even in populations for which no polymorphic markers have been ...

Searching the genome of beluga (Huso huso) for sex markers based on targeted Bulked Segregant Analysis (BSA)

In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we empl...

Combined bulked segregant sequencing and traditional linkage analysis for identification of candidate gene for purple leaf sheath in maize

Anthocyanin accumulation in various maize tissues plays important roles in plant growth and development. In addition, some color-related traits can be used as morphological markers in conventional maize breeding processes and purity identification of hybrid seeds. Here, we noticed that the leaf sheath color was controlled by a dominant gene, because purple (PSH) and green leaf sheaths (GSH) wer...

searching the genome of beluga (huso huso) for sex markers based on targeted bulked segregant analysis (bsa)

in sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. in the present study we empl...

RNA - seq and Bulked Segregant Analysis of a Gene Related to High Growth in 1 Ginkgo biloba Half - sibling families